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First, to extend the use of female sterilization : direct examination cotton swab take vagina, cervix or vaginal secretions walls white film, curettage biopsy male penile glans penis, or penile foreskin Department superficial lesions were performed as you specimens. Specimens will be used by 10% potassium hydroxide or saline producer, was found in flocks of egg-shaped spores and pseudohypha Park. If found more pseudomycelium, shows pathogenic Candida at the stage of diagnosis more meaningful.
Second, the inspections were also available : Gram staining, Congo Red staining or staining microscopy PAS staining. its high false-positive rate than direct. Gram staining, spores and pseudohypha stained red and blue : the PAS staining, and pseudohypha spores were stained red.
Third, the smear-negative patients : Isolation, culture for Candida. The specimens were examined under aseptic conditions in the medium Samaniego (the use of in vitro cultured). Tube media coverage will be a little steep overcoats, each tube were 2-3, were two specimens of each tube. 37 ＜ C temperature inside will be placed in incubation medium 24-48 hours after observation, we can see that a large number of white colony. smear small colonies were picked using needle directly or stained microscope examination, we can see that a large number of spores son. The initial diagnosis of candida infection.
Fourth, the use of immune-expansion or latex agglutination test can detect antibodies Candida albicans.
This article was posted on 2007-02-27
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